Force and compliance measurements on living cells using atomic force microscopy (AFM)
نویسندگان
چکیده
منابع مشابه
Atomic Force Microscopy indentation of living cells
Atomic Force Microscopy (AFM) indentation on living cells in physiological medium is a well established technique used for investigation of the cell mechanical properties at nanoscale. However, interpretation of the experimental data obtained in such experiments is rarely a trivial task. This is happening because living cells have a complex response to the applied mechanical stress, response th...
متن کاملCompliance measurements of confined polystyrene solutions by atomic force microscopy.
R. M. Overney,1 D. P. Leta,1 C. F. Pictroski,1 M. H. Rafailovich,2 Y. Liu,2 J. Quinn,2 J. Sokolov,2 A. Eisenberg,3 and G. Overney4 1Exxon Research and Engineering Company, 1545 Route 22 East, Annandale, New Jersey 08801 2Department of Materials Science, State University of New York at Stony Brook, Stoney Brook, New York 11794-2275 3Department of Chemistry, McGill University, Montreal, Canada H3...
متن کاملFinite Element Simulation of Contact Mechanics of Cancer Cells in Manipulation Based on Atomic Force Microscopy
The theory of contact mechanics deals with stresses and deformations which arise when the surfaces of two solid bodies are brought into contact. In elastic deformation contact occurs over a finite area. A regular method for determining the dimensions of this area is Hertz Contact Model. Appearance of atomic force microscope results in introduction of Contact ...
متن کاملAtomic Force Microscopy and Direct Surface Force Measurements
The atomic force microscope (AFM) is designed to provide high-resolution (in the ideal case, atomic) topographical analysis, applicable to both conducting and nonconducting surfaces. The basic imaging principle is very simple: a sample attached to a piezoelectric positioner is rastered beneath a sharp tip attached to a sensitive cantilever spring. Undulations in the surface lead to deflection o...
متن کاملRelative microelastic mapping of living cells by atomic force microscopy.
The spatial and temporal changes of the mechanical properties of living cells reflect complex underlying physiological processes. Following these changes should provide valuable insight into the biological importance of cellular mechanics and their regulation. The tip of an atomic force microscope (AFM) can be used to indent soft samples, and the force versus indentation measurement provides in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biological Procedures Online
سال: 2004
ISSN: 1480-9222
DOI: 10.1251/bpo67